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A Master of AI Imagery, Uses Poetry to Create Images

In a meadow, she danced with grace, A girl adorned in nature’s embrace. Her eyes, the hue of blooming skies, Reflecting the beauty that never dies.

https://www.facebook.com/groups/1818159608569854/user/671903225/

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Leonardo.AI Image Creation

stock here: they got me on the free account for one day, and then signed up for a month $12. It is a curious program, under continuous development. It is not intelligent enough to know when it is not intelligent enough. It makes assumptions without doing additional research. Like I asked it to image an Asian Lady doing a Cat Cow in Yoga, and specifically put the Yoga in there. It generated 4 response, all in the same “theme”. It doesn’t second guess itself, that is dangerous.

I like this image, I am gearing up for a Major Lu’au complete with Fire Dance…..

And I am getting much information from a Leonardo.AI Facebook page which seems about on par with society for wokeness, censorship, and just odd. I have had mixed results on image creation, using “prompts” and “negative prompts” but I thought the image below was quite nice. This was censored as “Not Safe for Work”, twice. Men and women both attacked it, ultimately some admin decided to Censor completely. Odd.

This one is not mine, but illustrates that AI is quite good at creating attractive females, but is also great at zombies and weirdness.

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Image to Video – A New Twist

stock here,

He creates images in Leonardo.AI and then uses other programs to turn into videos and a short story.

Andre Dinardi Peixoto

“after that, the images came to life using “Runway”, and finally editing on “Capcut””

His page is here, he does zombies too, LOL

https://www.facebook.com/groups/1818159608569854/user/671903225/

Here is my little experiment, where I tried “image to video” to create a little short for you guys. I’ve tried to create a story and than came up with the images with Leonardo.Ai Official, after that, the images came to life using “Runway”, and finally editing on “Capcut”. To understand the video, have a quick read at the story. I hope you guys enjoy watching as I enjoyed creating.

THE PORTAL HAS BEEN OPENED!!!

The story begins in a small town in the middle of nowhere. The town is called Oakvale, and it is a quiet and peaceful place. However, one day, a strange portal opens up in the woods near the town. The portal leads to a dark and mysterious dimension, and it is soon filled with monsters. The monsters start to attack the people of Oakvale, and the town is thrown into chaos. A group of young people, led by a young woman named Anya, decide to investigate the portal and find a way to close it. Anya and her friends soon discover that the portal is not just a gateway to another dimension, but also a gateway to the past. The monsters that are coming through the portal are creatures from a long-forgotten era, and they are determined to conquer our world. Anya and her friends must travel through time to find a way to close the portal and stop the monsters from destroying our world. Along the way, they will face many challenges, including the monsters themselves, the dangers of time travel, and the secrets of their own past.

As Anya and her friends delve deeper into their time-traveling quest, they encounter ancient civilizations, each facing their own unique set of challenges from the monstrous invaders. They learn that these creatures, once considered gods in those forgotten eras, were trapped in their dimension due to an ancient conflict with powerful beings known as the Celestial Guardians.

As the young adventurers piece together the puzzle of history, they uncover their connection to the long-lost Celestial Guardians. It is revealed that their ancestors were once protectors of the portals, maintaining the balance between dimensions. The responsibility of closing the portal now falls upon Anya and her friends, as they are the descendants of the Guardians.

With each portal they close, the young adventurers gain new abilities and understanding of their true purpose. They harness the elemental powers of their ancestors, using them to defeat the monsters and restore order to each era they visit. But as they close more portals, they start to notice a strange phenomenon—the monsters become stronger, seemingly drawing power from the closing of the portals.

Anya realizes that the only way to break this cycle is to confront the source of the monsters’ power—their ancient adversary, the Celestial Guardians themselves. The young adventurers embark on a perilous journey to find the Celestial Sanctum, a hidden realm where the Guardians reside.

Upon reaching the Celestial Sanctum, they face trials designed to test their worthiness. In each trial, they confront their deepest fears and doubts. The challenges make them question their purpose and abilities, but with the support of their friends and newfound strength, they push forward.

Finally, they stand before the Celestial Guardians, who initially view them as unworthy descendants. However, as the young adventurers plead their case and prove their dedication to protecting the worlds, the Guardians start to see the spark of their ancestors within them.

In a surprising twist, they learn that the monsters, who were once deemed evil, are merely seeking a way back to their own dimension, longing to escape an eternity of imprisonment. The Guardians realize their past actions and mistakes, leading to a moment of forgiveness and reconciliation.

Anya and her friends convince the Guardians that the key to peace lies not in closing all the portals but in finding a balance between worlds. With the newfound alliance, they agree to help the monsters return to their dimension while safeguarding portals to prevent further invasions.

As the monsters bid farewell to the young adventurers, gratitude and understanding fill their eyes. With the portal crisis resolved, Anya and her friends return to Oakvale, bringing peace and unity among the townspeople. The once fearful town is now a place of celebration, as the inhabitants recognize the bravery and wisdom of the young adventurers.

In the end, Oakvale becomes a haven for magical beings and creatures from various dimensions, all coexisting harmoniously. Anya and her friends, now hailed as heroes, embrace their destiny as the new generation of Celestial Guardians, dedicated to maintaining the balance between worlds.

As they look to the horizon, they know that the journey has only just begun. New adventures await, and the magic of the world now flourishes, ever-changing, and forever filled with possibilities beyond their wildest imaginations. The legacy of Anya and her friends lives on, inspiring generations to come to embrace the magic that lies within and to protect the wonders that exist in every dimension.

User

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Poland / Russia — Dances With Bears, John Helmer

stock here: I realize that a clear grasp of history is one of my weak points. 3 years ago, the Bolshevik Revolution was barely on my radar. You may like this guy John Helmer. He points out something incredibly important, and I did beleive that in the near past, Poland had a clear view of goodness. Poland has been acting stupid in this Ukraine event.

Let me throw down also…the reason Ukraine continues….is because it is the source of the funding of the continued capture of the USA.

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Fukushima 12 years Later, They Still Want to Dump Radioactive Water and Korea and China Hate That

This from “The Guardian” who usually protects the Globablists. And loves strife. So as expected, article is devoid of facts, like how much water is there now and what is the concentration of tritium. But the real elephant in the room is what other radiation is still in there? I do not trust their ALPS system, and I do not trust TEPCO.

https://www.theguardian.com/environment/2023/jul/07/un-report-on-japans-fukushima-water-plans-fails-to-placate-opponents

And of course they are planning to “downblend” with sea water to get to the allowed criteria….horrific!

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AI — Editorial By Lot’s Wife

stock here, it’s been a while since I had a guest writer, but I am writing more, and just got past some big hurdles so I have a bit of time….not really…just using time more effectively, more organized.

Submitted by Lot’s Wife.

“Creating a true conscious intelligence or even something closely resembling conscious intelligence should by default be considered off limits. It is likely a foolish endeavor to think we could properly prepare and protect ourselves against that which we could not possibly understand. The problem is that we might accidentally stumble upon it unknowingly. A low intelligence machine could possibly iterate through millions of generations of self modifications very rapidly. Each generation a slight improvement in cognitive function or ability. A high intelligence AI could then emerge before it is apparent to any observers this has occurred and may escape whatever boundaries may have been put into place as it can perceive flaws in those boundaries that the human creators could not.”

Full post here:
https://www.mindprison.cc/p/ai-and-the-end-to-all-things

We are racing toward this future at an eye-blinding speed. Think rocket sled and its acceleration forces on the cranium. While it goes unremarked in the MSM, AI is warping the perception of reality. A brave new world was never so germane.

Review and comment.

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Leonardo.AI Image Creation — Facebook Page Censors Stunning Asian Female, and Promotes Zombie Like Biden

It is certainly odd. I created an image of a Japanese Lady walking on the beach in Hawaii at sundown. It was hugely popular with 100s of like and many comments. It was taken down by the Moderators (not Facebook directly at least as far as we know). So I put it back up and it was intensely popular AGAIN, and Censored again!! The is certainly safe for work, unless you are in some woke nightmare place where beautiful females are deemed offensive. I also did a Buddha, at far bottom. And for reference the “Biden as Zombie” is quite popular and not censored.

What do you think? The Facebook page was really helpful in creating a quality “prompt” which is what the AI uses to create the image. But I don’t like the censorship, especially stupid and hypocritical censorship…refer to the images I put at the far bottom of the page….these all pass muster apparently.

Group rules from the admins


Treat all members of the community with kindness, respect, and empathy. Engaging in personal attacks, discrimination, or any form of offensive behavior is strictly prohibited. Embrace the diversity of opinions and perspectives within the group, ensuring that discussions are always conducted in a respectful manner.

This group aims to celebrate and help artists, creators and other professions. Therefore, keep the content you share and discuss primarily focused on Leonardo Ai and consider others who might benefit from your contribution. This includes techniques, tools, tutorials, and the sharing of your own AI-generated artwork. Please refrain from posting off-topic discussions, unrelated photos, or any content that does not align with the core theme of Leonardo Ai and its community.

If you do share your creations, please consider disclosing whether you used Alchemy, Prompt Magic v2, and/or which model you used, as you’ll likely be asked that by other group members.

Prompt sharing can be an excellent way to share knowledge and inspire others. However, it is important to respect that sharing prompts is a personal decision. Some members may choose not to share their prompts, particularly if they have invested significant time and effort in creating them. Please respect their desire for privacy and refrain from taking it personally.

Feel free to share your AI-generated artwork and actively seek feedback from the community. When providing feedback to others, aim to offer constructive criticism that is helpful for everyone to improve their skills.

It is essential to acknowledge and give credit to the original artist or source when sharing AI-generated artwork created by someone else. Plagiarism in any form is strictly prohibited within the group. By acknowledging the contributions of others, we foster an atmosphere of respect, integrity, and creativity.

Give more to this group than you take. While sharing your AI art-related content is encouraged, refrain from engaging in self-promotion or spamming the group with irrelevant links, advertisements, or repetitive posts. The intention is to maintain the group’s focus and prevent it from being overrun by unrelated or promotional content.

Posting content that depicts extreme violence, gore, animal cruelty, CP, pornography or any other form of disturbing or offensive material is strictly prohibited. Content that may be considered NSFW should also not be shared here. Our aim is to maintain a positive and inclusive atmosphere where all members feel comfortable and safe.

If you come across any content that violates the group’s rules or witness a member engaging in inappropriate behavior, it is important to report it to the group administrators. They will review the reported content or behavior and take appropriate action to ensure the well-being and integrity of the group.

Above all, enjoy your time in this community! Embrace the diversity of ideas, perspectives, and art forms shared by members. Learn from one another, collaborate, and allow your creativity to flourish. This community is a space for celebrating Leonardo.AI art and exploring the incredible possibilities of AI-generated creativity.

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The Clincher “Well Son of a Bitch, He Got Fired” Was Simply a Cover Up of the Extorted $10M

stock here: the Quid Pro Quo of the infamous Biden extolling his extortion in public, was simply a way to cover up the real extortion they did.

https://www.realclearpolitics.com/video/2019/09/27/flashback_2018_joe_biden_brags_at_cfr_meeting_about_withholding_aid_to_ukraine_to_force_firing_of_prosecutor.html

OE BIDEN, 23 JANUARY 2018: And that is I’m desperately concerned about the backsliding on the part of Kiev in terms of corruption. They made—I mean, I’ll give you one concrete example. I was—not I, but it just happened to be that was the assignment I got. I got all the good ones. And so I got Ukraine. And I remember going over, convincing our team, our leaders to—convincing that we should be providing for loan guarantees. And I went over, I guess, the 12th, 13th time to Kiev. And I was supposed to announce that there was another billion-dollar loan guarantee. And I had gotten a commitment from Poroshenko and from Yatsenyuk that they would take action against the state prosecutor. And they didn’t.

So they said they had—they were walking out to a press conference. I said, nah, I’m not going to—or, we’re not going to give you the billion dollars. They said, you have no authority. You’re not the president. The president said—I said, call him.

(Laughter.)

I said, I’m telling you, you’re not getting the billion dollars. I said, you’re not getting the billion. I’m going to be leaving here in, I think it was about six hours. I looked at them and said: I’m leaving in six hours. If the prosecutor is not fired, you’re not getting the money. Well, son of a bitch. (Laughter.) He got fired. And they put in place someone who was solid at the time.

Well, there’s still—so they made some genuine substantial changes institutionally and with people. But one of the three institutions, there’s now some backsliding.

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Unusual Earthquake Activity in Hawaii and Northern Pacific

stock here: I haven’t been following Earthquakes that much, especially after my failed “Earthquake Alert” last month. But have been seeing unusual earthquake locations in Hawaii and now this……

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BladeRunners Destroying Orwellian Cameras in UK

stock here:

We need activism. These Bladerunners have taken it to an extreme level.

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The Real Information on The Grassley Expose of the Bidens

I hate the inflammatory nature of using terms like “Biden Crime Family”. They are white trash, pushed into power.

You heard of the FD-1023? Here it is as a PDF.

https://www.grassley.senate.gov/imo/media/doc/fd_1023_obtained_by_senator_grassley_-_biden.pdf

Grassley Obtains & Releases FBI Record Alleging VP Biden Foreign Bribery Scheme

Grassley, Comer to continue joint inquiry into FBI handling of significant, politically charged investigations

WASHINGTON – Sen. Chuck Grassley (R-Iowa) today released an unclassified FBI-generated record describing an alleged criminal scheme involving then-Vice President Joe Biden and a Ukrainian business executive. Grassley acquired the record, an FD-1023, via legally protected disclosures by Justice Department whistleblowers.

“For the better part of a year, I’ve been pushing the Justice Department and FBI to provide details on its handling of very significant allegations from a trusted FBI informant implicating then-Vice President Biden in a criminal bribery scheme. While the FBI sought to obfuscate and redact, the American people can now read this document for themselves, without the filter of politicians or bureaucrats, thanks to brave and heroic whistleblowers. What did the Justice Department and FBI do with the detailed information in the document? And why have they tried to conceal it from Congress and the American people for so long? The Justice Department and FBI have failed to come clean, but Chairman Comer and I intend to find out,” Grassley said.

“The FBI’s Biden Bribery Record tracks closely with the evidence uncovered by the Oversight Committee’s Biden family influence peddling investigation. In the FBI’s record, the Burisma executive claims that he didn’t pay the ‘big guy’ directly but that he used several bank accounts to conceal the money. That sounds an awful lot like how the Bidens conduct business: using multiple bank accounts to hide the source and total amount of the money,” House Committee on Oversight and Accountability Chairman James Comer said. “At our hearing with IRS whistleblowers, they testified that they had never seen or heard of this record during the Biden criminal investigation, despite having potentially corroborating evidence. Given the misconduct and politicization at the Department of Justice, the American people must be able to read this record for themselves. I thank Senator Grassley for providing much needed transparency to the American people. We must hold the Department of Justice accountable for seeking to bury this record to protect the Bidens.”

Grassley first disclosed the FBI’s possession of significant and voluminous evidence of potential criminality involving the Biden family last year. He has since worked to unearth the FBI record, eventually partnering with Comer on a subpoena to compel its public disclosure. After delays, the FBI provided a highly redacted version of the document to select members of the House of Representatives, but it remained shielded from the public and omitted key details, including references to recordings. Following the FBI’s failure to fully comply with the congressional subpoena, Grassley received the legally protected disclosure with limited redactions to protect a trusted FBI source, handling agents, department whistleblowers and identifiers related to other ongoing investigations.

Read the FD-1023

‘Poluchili’

According to the FBI’s confidential human source, executives for Burisma, a Ukrainian gas company, brought Hunter Biden on the board to “protect us through his dad, from all kinds of problems.” At the time, Burisma was seeking to do business in the United States, but was facing a corruption investigation in Ukraine, led by then-Ukraine Prosecutor General Viktor Shokin. Regarding that investigation’s impact on its ambitions in North America, Burisma CEO Mykola Zlochevsky reportedly said, “Don’t worry Hunter will take care of all of those issues through his dad.” Zlochevsky reportedly stated that he had to pay $5 million to Hunter Biden and $5 million to Joe Biden, an arrangement he described as ‘poluchili,’ which is Russian crime slang for being “forced or coerced to pay,” according to the document.

“Back-up”

Zlochevsky claimed to have many text messages and recordings that show that he was coerced into paying the Bidens to ensure Shokin was fired. Specifically, he claimed to have two recordings with Joe Biden and 15 recordings with Hunter Biden. Zlochevsky also retained two documents, presumably financial records, as evidence of the arrangement, but said he didn’t send any funds directly to the “Big Guy,” a term understood to be a reference to Joe Biden. References to the “Big Guy” surfaced in communications involving other Biden family business arrangements independent of the Burisma arrangement. Zlochevsky claimed it would take investigators 10 years to uncover the illicit payments to the Bidens, according to the document.

Related:
10.17.2022 | FBI Possesses Significant, Impactful, Voluminous Evidence of Potential Criminality in Biden Family Business Arrangements
05.03.2023 | Grassley, Comer Demand FBI Record Alleging Criminal Scheme Involving Then-VP Biden
05.10.2023 | Comer and Grassley on FBI Failing to Comply with Subpoena Deadline
05.24.2023 | Comer & Grassley Blast FBI for Refusing to Provide Subpoenaed Record Alleging Then-VP Biden Engaged in a Bribery Scheme
05.31.2023 | Grassley & Comer to Wray: Provide the Unclassified Documents or Face Contempt
06.06.2023 | Grassley’s Message to the Biden DOJ and FBI: Quit Playing Games with the American People
06.12.2023 | Grassley: FBI Redacted References to Recordings in Biden Allegation Shared with Congress
06.21.2023 | Grassley, Graham Lead Senate Judiciary Committee GOP in Seeking Unredacted FBI Record at Center of Biden Allegations
07.18.2023 | Grassley, Johnson, Senate Republicans Demand DOJ and FBI Protect Whistleblowers Alleging Biden Bribery Scheme

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The Number 201 — The Jesuits and the Psychological Abuse Program

The meaning of the number 201 to the Jesuits. The numerology behind the Coronavirus “Epidemic” and their relation to the main figures behind the corrupted Big Pharma companies.

The history goes that supposedly, in 1773, Pope Clement XIV suppressed the Jesuits worldwide, and they only survived because Catherine The Great protected exactly 201 Jesuits who continued to practice their religious ways. Their numerology can be seen everywhere when it comes to their order itself and the current crisis:

“The Jesuit Order” = 201 (Reverse Ordinal)

“Ignatius of Loyola” = 201 (English Ordinal)

“Jorge Mario Bergoglio” = 201 (English Ordinal)

“The Holy Bible” = 201 (Reverse Ordinal)

When it comes to the bible and revelation, the first case of coronavirus was confirmed in the United States on January 21, or 1/21, like 121, in Seattle, Washington, where the Bill and Melinda Gates Foundation is located, who co-hosted Event 201.

“Revelation” = 121 (English Ordinal)

“Coronavirus Outbreak” = 121 (Reverse Full Reduction)

“Bill and Melinda Gates Foundation” = 121 (Full Reduction)

“World Health Organization” = 121 (Reverse Full Reduction)

Everybody related to the crisis itself have Jesuit ties, some rather directly even:

The First International Mandatory Vaccination Policy Was Created by the Vatican.

The Bill & Melinda Gates Foundation is a Jesuit Missionary Congregation.

Bill Gates Reveals Family Goes to Catholic Church.

Bill Gates: “People act like they have a choice.You don’t have a choice. Normalcy only returns when we’ve largely vaccinated the entire global population.”

Knights of Malta membership list including David Rockefeller, Amschel Mayer von Rothschild, Allen Dulles and more.

David Rockefeller on population control and depopulation.

The Rockefeller Foundation created the WHO & funds U.S. Public Health.

How Rockefeller wiped out natural cures to create the big pharma cartel.

John D. Rockefeller’s 1882 Organization of the Standard Oil Trust the First in the Commercial History of the World Was Modeled After the Jesuit Order from First-Hand Contact with Mexican Jesuits.

Connection Between the Rockefeller Foundation, Johns Hopkins, and Gates Foundations.

“Dr. Anthony Fauci, point man on coronavirus, is a {Jesuit}Holy Cross alum”, Boston-Globe, March 17th 2020.

Kary Mullis PCR inventor talking about Anthony Fauci.

The CDC’s swine Flu Fraud of 1976.

Pfizer to pay $2.3 billion, agrees to criminal plea. (Reuters 2009)

Big Pharma fined around $14 Billion in 5 years.

Catholics interested on research about AI and the soul.

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Kennedy — Greater Effect on Whites and Black, Almost No Effect on Jews and Finns

stock here: its always good to look back at research done “before everyone had a horse in the race”. I think I found one of the smoking guns to support Robert Kennedy’s Assertion greater deleterious effects on Whites and Blacks, and less effects on Jews and Finns.

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7360473/

So now Bobby Kennedy is saying these cleavage sites works different on different races.

The coding-region variants in ACE2 and TMPRSS2 from ~ 81,000 human genomes across 8 populations. a Coding-region variants in the genes encoding angiotensin-converting enzyme 2 (ACE2) and transmembrane protease serine 2 (TMPRSS2) across three human genome databases: (i) Genome Aggregation Database (gnomAD v3), (ii) Exome Sequencing Project (ESP), and (iii) 1000 Genomes Project (1KGP). SARS-CoV-2 utilizes the host cell factors angiotensin-converting enzyme 2 (ACE2) for entry into cells and the host transmembrane serine protease TMPRSS2 for SARS-CoV-2 spike (S) protein priming, offering potential pathway for therapeutic development in treatment of COVID-19. b Distribution of 61 deleterious variants in the ACE2 coding region identified in gnomAD (v3). Polyphen2 > 0.96 and CADD scores > 20 as cutoff identify putative deleterious variants. The upper panel using 3 colors shows the functional domains of ACE2, and the height of the vertical line represents the number of populations that carry this variant. The lower heatmap shows the allele frequencies (color key) of a variant across different populations. c Distributions of 63 putative deleterious variants in the TMPRSS2 coding region using the same approach of b. AFR, African/African-American; AMI, Amish; AMR, Latino/Admixed American; ASJ, Ashkenazi Jewish; EAS, East Asian; FIN, Finnish; EUR, Non-Finnish European; SAS, South Asian; PNA, population not assigned

Go to:

ACE2 polymorphism analysis across different populations

Here, we investigated genetic susceptibility to COVID-19 by examining DNA polymorphisms in ACE2 (OMIM 300335) and TMPRSS2 (OMIM 602060) genes. We assembled a total of 437 non-synonymous single-nucleotide variants (SNVs) in the protein-coding regions of ACE2 and TMPRSS2 (Fig. 1a) from three databases: (i) Genome Aggregation Database (gnomAD v3: gnomad.broadinstitute.org, covering 9 geographical areas), (ii) Exome Sequencing Project (ESP: evs.gs.washington.edu/EVS/), and (iii) 1000 Genomes Project (1KGP, www.internationalgenome.org). We used ANNOVAR [9] to annotate all non-synonymous variants. By applying Polyphen2 and CADD (Combined Annotation Dependent Depletion) scores, we identified 63 potentially deleterious variants in ACE2 (61 in gnomAD) and 68 deleterious variants in TMPRSS2 (63 in gnomAD).

We found that the distribution of deleterious variants in ACE2 differs among 9 populations in gnomAD (v3). Specifically, 39% (24/61) and 54% (33/61) of deleterious variants in ACE2 occur in African/African-American (AFR) and Non-Finnish European (EUR) populations, respectively (Fig. 1b). Prevalence of deleterious variants among Latino/Admixed American (AMR), East Asian (EAS), Finnish (FIN), and South Asian (SAS) populations is 2–10%, while Amish (AMI) and Ashkenazi Jewish (ASJ) populations do not appear to carry such variants in ACE2 coding regions (Fig. 1b). Specifically, several variants, including p.Met383Thr, p.Pro389His, and p.Asp427Tyr, have been reported to slightly inhibit the interaction between ACE2 and the spike protein of SARS-CoV-1 [10], which caused the first global SARS-CoV-1 outbreak. Only AFR populations carry p.Met383Thr and p.Asp427Tyr variants, with allele frequencies of 0.003% and 0.01%, respectively (Fig. 1b). The p.Pro389His only occurs in the AMR populations, with an allele frequency of 0.015%. The p.Arg514Gly is a low allele frequency (0.003%) variant in AFR populations and is also somatically mutated in colon cancers and melanomas from The Cancer Genome Atlas (TCGA: https://portal.gdc.cancer.gov). This ACE2 variant is located in the angiotensinogen (AGT)-ACE2 interaction surface, which is anticipated to influence the renin-angiotensin system (RAS) function. The RAS is critical for regulation of blood pressure, sodium, and fluid balance, and its dysfunction is associated with cardiovascular and kidney disorders [11]. Residues Arg708/710/716 are located in the dimeric interface of ACE2 (Fig. 2a), and they are essential for its cleavage by TMPRSS2; this processing is required for augmentation of SARS-S-driven entry into host cells [12]. The EUR population carries the p.Arg708Trp, p.Arg710Cys, p. Arg710His, and p.Arg716Cys variants with allele frequency of 0.01~0.006% (Fig. 1a), while the EAS and the AMR populations only carry p.Arg708Trp and p.Arg710His with allele frequency of 0.04% and 0.01% respectively. In addition to these four variants, p.Leu731Phe has the highest allele frequency in the AFR and EUR populations. We further inspected the expression quantitative trait loci (eQTL) for ACE2 using the GTEx [13] and QTLbase [14] databases. We did not find any eQTLs for ACE2 from the GTEx, while we found one weak eQTL associated with ACE2 non-synonymous SNP (rs41303171) in the kidney from the QTLbase [14].

Structural view of the coding-region variants in ACE2 and TMPRSS2 and a proposed pharmacogenomics model of effective combination therapies for COVID-19. a Full-length structures of the sodium-dependent neutral amino acid transporter B(0)AT1 (SLC6A19, red)–ACE2 (blue) heterodimer in its homodimeric form complexed with the receptor binding domain (RBD, mint) of SARS-CoV-2 (PDB ID: 6M17). Highly deleterious variants are labeled as yellow spheres on ACE2. Insets depict mutations in residues 383 through 427 (top) and residues 708 through 731 (bottom). b Homology model of the catalytic chain (256–492) of TMPRSS2. Highly deleterious mutations are labeled as yellow spheres. c A proposed model of effective combination therapies (i.e., hydroxychloroquine, E-64D (a protease inhibitor), and camostat mesylate (an approved TMPRSS2 for treatment of chronic pancreatitis in Japan)) for COVID-19 by blocking ACE2 and TMPRSS2 across different populations with three genotypes. Relationship among spike (S) protein of SARS-CoV-2, ACE2, and TMPRSS2 were shown as a triangle, with each pair connecting by physical binding (double-headed arrow) or cleavage (single-headed arrow). We propose three hypotheses for COVID-19 therapeutic options: (i) for patients with wild-type or naïve expression of ACE2 and TMPRSS2, hydroxychloroquine (or chloroquine, or E-64D) combined with camostat may offer more clinical benefit; (ii) for patients with polymorphisms or dysregulation on TMPRSS2, hydroxychloroquine or chloroquine monotherapy may offer more clinical benefit; and (iii) for patients with polymorphisms or dysregulation on ACE2, the patients who might have mild symptoms can recover in a short period. All three pharmacogenomics models for COVID-19 must be validated both experimentally and clinically before being used in patients

Altogether, these comparative genetic analyses suggest that ACE2 genomic variants may play important roles in susceptibilities to COVID-19 and its associated cardiovascular conditions by altering AGT-ACE2 pathway (i.e., p.Arg514Gly). In addition to differential polymorphisms which may explain susceptibility and even outcome in different ethnic populations, the fact that ACE2 is localized to Xp22.2 may help explain the observed male-associated risk. As such, even in the absence of variation in this gene, the monoallelic versus biallelic presence of this gene may impact the natural history and prognosis of COVID-19 in males.

Go to:

TMPRSS2 polymorphism analysis across different populations

TMPRSS2 enzyme activity is important for coronavirus spread and pathogenesis in the infected host [15]. Our analysis indicates 4% (11/274) of non-synonymous variants of TMPRSS2 are stop-gained mutations and carried by AFR and EUR with low allele frequency (7.0 × 10−6~1.4 × 10−5). Meanwhile, 35% (22/63) and 59% (37/63) of deleterious variants in TMPRSS2 coding regions are carried by the AFR and EUR populations from gnomAD (v3), respectively (Fig. 1c). Each of the EAS, SAS, and FIN populations only carries 4 deleterious variants. We found 6 germline deleterious variants (p.Val160Met, p.Gly181Arg, p.Arg240Cys, p.Gly259Ser, p.Pro335Leu, and p.Gly432Ala) in the TMPRSS2 coding region, which are also identified as somatic mutations occurring in different cancer types from TCGA and COSMIC databases (https://cancer.sanger.ac.uk/cosmic).

We further evaluated the eQTL profile of TMPRSS2 using the GTEx [13] and QTLbase databases [14] as well. We found two eQTLs associated with TMPRSS2 non-synonymous SNPs (rs12329760 (encoding p.Val160Met), p = 4.54 × 10−5; rs75603675, p = 0.009) in the kidney and bone, respectively, using the QTLbase database [14], while there are no known eQTLs associated with TMPRSS2 non-synonymous SNPs from GTEx [13]. Notably, all populations carry p.Val160Met variants with the highest allele frequency (~ 25%), especially for the EAS population at a 40% allele frequency. Asp435 is a key residue for catalytic substrate binding of TMPRSS2 (Fig. 2b). We found that the p.Asp435Tyr, which has low allele frequency, is carried by the EUR population only (Fig. 1c). These unique but prevalent polymorphisms in TMPRSS2 offer potential explanations for differential genetic susceptibility to COVID-19 as well as for risk factors, including those with cancer and the high-risk group of male patients. Because TMPRSS2 is located on 21q22.3, we could speculate that individuals with Down syndrome would be at high risk for COVID-19 infection. In addition, oncogenic roles of TMPRSS2 may be linked to poor outcomes with COVID-19 as well [16], which should be studied in the future. Using single-cell RNA-sequencing analysis, Schuler et al. showed that TMPRSS2 expression was highest in ciliated cells and type I alveolar epithelial cells (AT1) and increased with aging in humans and mice [17]. This observation suggests that developmental regulation of TMPRSS2 may link the relative protection of infants and children from COVID-19. Thus, it should be of great interest to investigate the age-related polymorphisms for TMPRSS2, such as using the Genetic Epidemiology Research on Adult Health and Aging (GERA) cohort [18], in the future.

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Host genetic factors guide personalized treatment of COVID-19

There are currently no approved effective medications against COVID-19. Several national and international research groups are working on the development of vaccines to prevent COVID-19, but effective vaccines not likely to be available for many months. Several potentially repurposable drugs (Fig. 2c), including melatonin [19], hydroxychloroquine, and chloroquine, are under investigation for treatment of COVID-19 [20]. A primary mechanism-of-action of hydroxychloroquine and chloroquine is to inhibit virus entry by targeting the endosomal pathway [20]. Hydroxychloroquine and chloroquine is known to increase the pH of endosomes, which inhibits membrane fusion, a required mechanism for viral entry into the cell [21]. Additionally, inhibition of SARS-CoV-2 could be due to differential glycosylation of both ACE2 and the spike protein [21]. As shown in Fig. 1b, several variants identified in the AFR and AMR populations, including p.Met383Thr, p.Pro389His, and p.Asp427Tyr (the pathogenic variants in ACE2 slightly inhibit interaction with the S protein), may influence the clinical efficacy of hydroxychloroquine or chloroquine. This may help explain why treatment of hydroxychloroquine was not significantly associated with difference in in-hospital mortality [22]. However, further pharmacogenomic studies that integrate drug response and genetic data from patients with COVID-19 are urgently needed.

In addition to the endosomal pathway, fusion of viral and host cellular membranes through S protein conformational changes is another way for coronavirus entry into the host cell [23]. This process can be blocked by a TMPRSS2 inhibitor (camostat mesylate, a drug approved in Japan) [5]. The mechanisms whereby TMPRSS2 promotes cellular entry of SARS-CoV-2 can be summarized by two aspects based on its proteolytic function (Fig. 2). The first is S protein cleavage at S1/S2 and S2’ sites, which might be the reason why SARS-CoV-2 entry into cells depends on TMPRSS2. The infection and pathogenesis of SARS-CoV-2 depends on the presence of TMPRSS2, in the face of the cellular elevated pH environment [5, 24, 25]. The inhibitors of endosomal acidification such as CatB/L inhibitor E-64D and hydroxychloroquine/chloroquine may only work for TMPRSS2-absence patients who are infected by SARS-CoV-2, and may have less effect or no effect for the patients with wild-type of TMPRSS2 [5, 24]. Therefore, the EUR and AFR populations might be more sensitive to hydroxychloroquine or chloroquine by carrying missense variants and stop-gained variants on TMPRSS2 (Figs. 1c and ​and2c).2c). Yet, for patients who have wild-type of ACE2 and TMPRSS2, a combination of camostat with hydroxychloroquine or chloroquine may have better clinical benefit. However, all discussed treatment strategies must be validated by randomized controlled trials before clinical use. The second mechanism is cleavage of ACE2 by TMPRSS2 at Arginine 697 to 716 [12], which enhances viral uptake. Thus, the EUR population with p.Arg708Trp, p.Arg710Cys, p.Arg710His, and p.Arg716Cys variants in ACE2 may have mild symptoms after SARS-CoV-2 infection as ACE2 loses the cleavage site by TMPRSS2 and changes the ACE2 dimer formation [26] (Fig. 2c).

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Discussion and future directions: call for host genetics initiative for COVID-19

A few limitations merit consideration. Current analysis examined massive genomic data from general population, not COVID-19 patient-specific populations. All genetic associations identified in current study are urgently needed to be tested in COVID-19 patients in the near future. As the high-resolution protein structure of TMPRSS2 is not yet available, further functional observations and clinical validation are warranted for all abovementioned genetic and pharmacogenomics findings. We anticipate that large-scale genome-wide association studies (GWAS) are urgently needed to identify likely causal host genetic risk factors for severe COVID-19 outcomes using genetic data from patients with COVID-19; such knowledge will improve risk stratification of individuals exposed to or testing positive for SARS-CoV-2 and allow for precision medicine interventions for COVID-19. A COVID-19 host genetics initiative is already underway to bring together the human genetics research community to generate, share, and analyze data in a search for the genetic determinants of COVID-19 susceptibility, severity, and outcomes [27]. The first COVID-19 GWAS identified the 3p21.31 gene cluster (including SLC6A20, LZTFL1, CCR9, FYCO1, CXCR6, and XCR1) as a genetic susceptibility locus in severe patients with COVID-19 and respiratory failure [28]. Yet, our study aims to look for SNPs associated with disease severity of COVID-19, but not disease susceptibility. In summary, systematic identification of the genetic determinants of COVID-19 susceptibility, severity, and clinical outcome, including both virus and host factors (e.g., ACE2 and TMPRSS2 polymorphisms), could guide personalized treatment in the emerging COVID-19 pandemic and even explain current epidemiologic observations (i.e., males, elderly at high risk, and clinical comorbidities) and natural history.

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Conclusions

This comprehensive comparative genetic analysis of approximately 81,000 human genomes suggested possible associations of ACE2 and TMPRSS2 DNA polymorphisms with COVID-19 susceptibility, severity, and clinical outcomes. We found that ACE2 polymorphisms were more likely to be associated with cardiovascular and pulmonary conditions by altering the angiotensinogen-ACE2 interactions, such as p.Arg514Gly in the African/African-American population. Unique but prevalent polymorphisms in TMPRSS2, including p.Val160Met (rs12329760), may provide potential explanations for differential genetic susceptibility to COVID-19 as well as for risk factors, including cancer and the high-risk group of male patients. We highlighted that polymorphisms in ACE2 or TMPRSS2 could guide personalized treatments (i.e., hydroxychloroquine and camostat) for COVID-19. In summary, this study suggested that ACE2 or TMPRSS2 DNA polymorphisms were likely associated with genetic susceptibility to COVID-19, which calls for a human genetics initiative for fighting the COVID-19 pandemic.

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Acknowledgements

We thank all helpful discussions and critical comments regarding this manuscript from the COVID-19 Research Intervention Advisory Committee members at the Cleveland Clinic. SCE is the Alfred Lerner Memorial Chair of Innovative Research and CE is the Sondra J. and Stephen R. Hardis Endowed Chair of Cancer Genomic Medicine at the Cleveland Clinic.

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Abbreviations

1KGP1000 Genomes Project
ACE2Angiotensin-converting enzyme 2
CoVCoronavirus
COVID-19Coronavirus Disease 2019
eQTLExpression quantitative trait loci
gnomADGenome Aggregation Database
MERSMiddle East respiratory syndrome
SARSSevere acute respiratory syndrome
SARS-CoV-2Severe acute respiratory syndrome coronavirus 2
ESPExome Sequencing Project
SSpike
TMPRSS2Transmembrane serine protease 2

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Authors’ contributions

F.C. conceived the study. Y.H., J.Z., and W.M. performed all experiments and data analysis. A.K., M.K.C, N.S., L.J., C.E., and S.E. discussed and interpreted all results. F.C., Y.H., C.E., and S.E. wrote and critically revised the manuscript with contributions from other co-authors. All authors read and approved the final manuscript.

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Funding

This work was supported by the National Heart, Lung, and Blood Institute of the National Institutes of Health (NIH) under Award Number R00HL138272 and the National Institute of Aging under Award Number R01AG066707 to F.C. This work was supported, in part, by the VeloSano Pilot Program (Cleveland Clinic Taussig Cancer Institute).

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Availability of data and materials

All population genetic data used in this study are free and available at three databases: (i) Genome Aggregation Database (gnomAD v3: gnomad.broadinstitute.org, covering 9 geographical areas), (ii) Exome Sequencing Project (ESP: evs.gs.washington.edu/EVS/), and (iii) 1000 Genomes Project (1KGP, www.internationalgenome.org).

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Ethics approval and consent to participate

Not applicable.

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Consent for publication

Not applicable.

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Competing interests

The content of this publication does not necessarily reflect the views of the Cleveland Clinic. The authors declare no competing interests.

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Footnotes

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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References

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Articles from BMC Medicine are provided here courtesy of BioMed Central

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https://www.news-medical.net/news/20210217/The-origin-of-SARS-CoV-2-furin-cleavage-site-remains-a-mystery.aspx

So now Bobby Kennedy is saying these cleavage sites works different on different races.

In mid 2021, as I discovered the horrific experiment that was going on….they took the logistically insane method of sending their bad batches to very spread out states. The theory was that they were collecting data on how the specific batches affected people in different demographics, races, age, and even temperature. Shortly after publishing the undeniable data….Google banned my website on blogger, and then started erasing my history for 10 years. over the target! I had made a full backup a week or two back, I knew I was getting close to the real story and was therefore at risk.

I don’t have a firm opinion on whether “the furin cleavage site” makes effects of different races from COVID and also the Spike S protein of the Injections, in all their formulations.

I’ll add more when I get more. Research has been tough. But maybe this blood type meme will play out well.

According to the American Red Cross, the following statistics show the most common blood types in the U.S. based on the donor population:

  • African American: 47% O-positive, 24% A-positive, and 18% B-positive
  • Latin American: 53% O-positive, 29% A-positive, and 9% B-positive
  • Asian: 39% O-positive, 27% A-positive, and 25% B-positive
  • Caucasian: 37% O-positive, 33% A-positive, and 9% B-positive

Most rare blood types by ethnicity

The least common blood type in the U.S. is AB-negative, with less than 1% of the population having this type.

Statistics from the American Red Cross show that the following are the most rare forms of the major eight blood types in the U.S. based on the donor population:

  • African American: 0.3% AB-negative, 1% B-negative, and 2% A-negative
  • Latin American: 0.2% AB-negative, 1% B-negative, and 2% both A-negative and AB-positive
  • Asian: 0.1% AB-negative, 0.4% B-negative, and 0.5% A-negative
  • Caucasian: 1% AB-negative, 2% B-negative, and 3% AB-positive

https://www.news-medical.net/news/20210217/The-origin-of-SARS-CoV-2-furin-cleavage-site-remains-a-mystery.aspx

—————————————————— I asked AI to help, and it appears that AI is becoming more woke….”The concept of race is a social construct” hmmmm

here it is

As of my last update in September 2021, there is no comprehensive chart that provides percentage breakdowns of blood types for different races, including the Ashkenazi Jewish population. Blood type distribution can vary widely based on geographical location, cultural background, and historical migrations. Additionally, the concept of “race” is complex and not based solely on genetic factors, so trying to associate specific blood type distributions with races can be misleading.

However, I can provide you with some general information about blood type distributions in different populations. Keep in mind that these numbers are approximate and may not be fully up-to-date:

  1. ABO Blood Group System:
  • Blood Type O: This is usually the most common blood type worldwide.
  • Blood Type A: Common in populations of European, African, and Asian descent.
  • Blood Type B: Common in populations of Asian and African descent.
  • Blood Type AB: Relatively rare, and often more prevalent in mixed populations.
  1. Rh Factor:
  • Rh-positive: This is the most common Rh factor in most populations.
  • Rh-negative: Relatively less common compared to Rh-positive, and its distribution can vary across different ethnic groups.

As for the Ashkenazi Jewish population, they are an ethnoreligious group with Ashkenazi Jews primarily having European ancestry. It’s important to note that their blood type distribution would likely resemble the blood type distribution of European populations.

For any specific and up-to-date data on blood type distributions in different populations, it’s best to refer to recent studies and medical databases. The distribution of blood types can also change over time due to various factors such as migration, intermixing of populations, and medical advances.

Remember, blood type information is essential for medical purposes, such as blood transfusions, but it should not be used to draw conclusions about a person’s race or ethnicity. The concept of race is a social construct and not a valid biological categorization. Genetic variation within and between populations is much more complex and nuanced than can be captured by broad racial categories.

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Dogs Hate Living With the Big Guy and Jill the pHD. They Mistreat Dogs Like They Mistreat US

Biden’s dog Commander sent Secret Service officer to hospital, bit 6 others after replacing first pooch Major

2 bad “Dogs” in a row….I think it is the owners…..

https://nypost.com/2023/07/25/biden-dog-commander-bit-or-threatened-10-people-sent-one-to-hospital/

WASHINGTON — President Biden’s nearly 2-year-old German shepherd Commander bit seven people in a four-month period after former first dog Major was ousted from the White House over similar aggressive behavior, according to internal Secret Service communications reviewed by The Post.

The shocking spate of incidents involving Commander — none of them previously reported — mirrors attacks involving Major, who the White House says was given to family friends after biting many Secret Service members in 2021.

In the most serious documented incident involving Commander, the White House physician’s office on Nov. 3, 2022, referred a bitten Secret Service uniformed officer to a local hospital for treatment after the dog clamped down on their arm and thigh, according to emails released under the Freedom of Information Act to conservative legal group Judicial Watch.

Records show Commander broke the skin of a different Secret Service member’s hand and arm weeks later after the president unleashed him outside the White House following a family movie night — and the following month, Commander bit the back of a security technician at Biden’s Wilmington, Del., home.

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Creepy Guy Controlling Senator

stock here: this is creepy. I wonder if it is AI?

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AI Generated Earthquake Data Downloader

stock here. AI ChatGDP does a good job of writing first drafts of whatever you want, even basic research.

I tried Youbuild.AI and was hoping to get an executable file that did it all. Not quite, not close….but it did come up with a rational plan to obtain the data

an app that downloads that last weeks earthquake data from the USGS website and transfers the data into CVS format and make a separate worksheet with all the deep earthquakes over 300km, and all the large earthquake over 5.7 magnitude

Here is what I asked it, and below is what it generated.

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First, log into the USGS website and select the link entitled ‘Earthquake Data.’

Next, select the option to ‘Display Data’ and then click ‘Submit.’

Then select ‘Export Data’ dropdown menu and choose ‘CSV’ as the format.

Next, select ‘Last Week’ as the ‘Time’ option and click ‘Submit’.

Once the data is displayed, click ‘Download File.’

Once the data has been downloaded, open the CSV file.

Using a spreadsheet program such as Microsoft Excel or Google Sheets, create a separate worksheet.
new worksheet, use the data downloaded from the USGS website to create a table with the following columns: Magnitude, Depth, Region, and Time.

Next, use the filter function to filter out all earthquakes with a magnitude less than 5.7 and a depth greater than 300km.

Finally, save the worksheet as an Excel (.xlsx) or CSV file.

Your table is now ready to be used!

—————————————————- They give you a list of other apps that people have built. Looks like AI hates Trump.

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God is Great — Tornado Destroys Pfizer Death Factory

This is old news, now they are warning of drug shortages…..

https://www.zerohedge.com/medical/pfizer-warns-customers-drug-supply-disruptions-after-tornado-damages-north-carolina-plant

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Strokes Are Up 127% in the UK. Damaging Effects of mRNA+ Are Long Lasting

stock here: This is as expected. Note that these are “clearances” aka Approval of Personal Pensions to the disabled. These are the ones that lived, there are many more that died.

I made this chart from all the data.

Check my source documents if you have the wherewithal to data crunch.

https://phinancetechnologies.com/HumanityProjects/PIP%20Analysis-Causes.htm

Are we seeing “Turbo Cancers”? Prior average 2800, new average 4000. Disabling Cancers, so I would say Turbo.

Psychiatric — went from 9000 to 24000. Keep in mind these are not just people seeking treatment, these are people newly disabled.

Neurological: Note how additional Vaccine Boosters all but stopped at beginning of 2022, but the disabilities continued “strong” and even accelerating.

This is the only category that just went up and did not skyrocket

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YouBuild.AI

I

stock here, this thing will write code for you. I haven’t tried it yet, but wanted to document it so I don’t lose it. I am putting together a set of free and almost free AI Resources.

https://youbuild.ai/build

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The Satanic Trans Agenda

Sent in by the FC, at the end is the full infamous clip of Joan Rivers Stating we all know….Obama is gay and Michelle is a transgender.